◆Purpose of the experiment: To test the preservation effect of cultured sugar powder APN-F2 in tomato sauce (stored at 25℃)
◆Experimental groups: Prepare cultured sugar powder APN-F2 solutions of different concentrations
The blank group does not add cultured sugar powder APN-F2
The positive control group adds 0.5% potassium sorbate (this: APN-F2 is white powder, which is dissolved in water to make a solution.)
Sensory Evaluation
| Sample | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 |
| Blank group | No pungent smell, strong tomato flavor | No pungent smell, strong tomato flavor | Slight pungent smell, slight odor | Slight pungent smell, slight odor | Slight pungent smell, obvious odor |
| 0.2% | Slightly pungent smell, strong tomato flavor | Slightly pungent smell, strong tomato flavor | Slight pungent smell, slight odor | ||
| 0.4% | No pungent smell, strong tomato flavor | ||||
| 0.6% | |||||
| 0.8% | Slightly pungent smell, strong tomato flavor | ||||
| 1.0% | |||||
| 1.2% | |||||
| Control group | |||||
★ Based on the above experimental results, it can be concluded that the optimal concentration of cultured sugar powder APN-F2 for extending the shelf life of tomato sauce without affecting the flavor of tomato sauce is 0.60%.
Colony Count(CFU/ml)
| Sample | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 |
| Blank group | 36±1.24 | 4.15×102 | 7.33×103 | 6.37×104 | 4.39×106 |
| 0.4% | 3.06×102 | 3.55×103 | 3.58×104 | 3.08×106 | |
| 0.6% | 2.78×102 | 2.94×103 | 3.09×104 | 2.25×106 | |
| 0.8% | 1.96×102 | 2.21×103 | 2.74×104 | 1.98×106 | |
| 1.2% | 1.34×102 | 1.16×103 | 1.86×104 | 1.39×106 | |
| 1.6% | 0.92×102 | 0.73×103 | 1.54×104 | 0.77×106 | |
| Control group | 3.11×102 | 3.70×103 | 3.44×104 | 3.23×106 |
★When the concentration of cultured sugar powder APN-F2 is higher than 0.60%, it can effectively extend the shelf life of tomato sauce.
The above experimental results show that, combined with the sensory evaluation results, when the concentration of cultured sugar powder APN-F2 is 0.6%, tomato sauce can be stored at 25°C for 5 days.
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