◆Test samples: cultured sugar powder APN-F1, APN-F2, cultured sugar SH, ascorbic acid, DPPH reagent, 95% ethanol, anhydrous ethanol
◆Experimental groups:
Sample group: 1ml sample solution + 2ml0.1mmol/L DPPH
Control group: 1ml anhydrous ethanol + 2ml0.1mmol/L DPPH
Blank group: 1ml sample solution + 2ml anhydrous ethanol
Figure 1 DPPH free radical scavenging rate of cultured sugar products (powder) at different concentrations
| Group | A sample | A blank | A control | DPPH radical scavenging rate | |
| Cultured sugar powder APN-F1 | 0.4% | 0.797 | 0.527 | 0.959 | 71.85% |
| 0.8% | 0.776 | 0.513 | 0.959 | 72.58% | |
| 1.2% | 0.783 | 0.490 | 0.959 | 69.45% | |
| 2.0% | 0.757 | 0.436 | 0.959 | 66.53% | |
| Cultured sugar powder APN-F2 | 0.4% | 0.802 | 0.521 | 0.959 | 70.70% |
| 0.8% | 0.776 | 0.510 | 0.959 | 72.26% | |
| 1.2% | 0.767 | 0.515 | 0.959 | 73.72% | |
| 2.0% | 0.746 | 0.463 | 0.959 | 70.49% | |
| Cultured sugar product SH | 0.4% | 0.902 | 0.505 | 0.959 | 65.31% |
| 0.8% | 0.862 | 0.525 | 0.959 | 65.94% | |
| 1.2% | 0.887 | 0.532 | 0.959 | 56.76% | |
| 2.0% | 0.834 | 0.442 | 0.959 | 49.74% | |
| Ascorbic acid | 1.0% | 0.479 | 0.429 | 0.959 | 94.79% |
Changes in DPPH free radical scavenging rate of cultured sugar products (powder) at different concentrations
Experimental conclusion
In general, the DPPH free radical scavenging rate of cultured sugar powder APN-F1 and APN-F2 is higher than that of cultured sugar product SH. Compared with ascorbic acid, because ascorbic acid has strong antioxidant properties, the DPPH free radical scavenging rate reaches 94.79% at a concentration of 1.0%.
Free
sample
